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High Affinity Orai Blockers Synta 66, supplied by Synta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the <t>Orai1</t> blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.
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Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the <t>Orai1</t> blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.
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Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the <t>Orai1</t> blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.
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Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the <t>Orai1</t> blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.
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Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the <t>Orai1</t> blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.
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Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the Orai1 blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.

Journal: Blood Advances

Article Title: Supramaximal calcium signaling triggers procoagulant platelet formation

doi: 10.1182/bloodadvances.2019000182

Figure Lengend Snippet: Role of SOCE in procoagulant platelet generation. To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the Orai1 blocker Synta-66 (10 µM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry. Representative density plots are shown for Fluo-4–loaded platelets (A) or Fluo-5N–loaded platelets (B). (C) The percentage of platelets that bound AnV (***P < .001; paired Student t test). The effect of Synta-66 on Fluo-4 (D) or Fluo-5N (E) fluorescence in all platelets, AnV+, platelets and AnV– platelets. *P < .05 for dimethyl sulfoxide (DMSO) vs Synta-66–treated platelets; 2-way analysis of variance with the Šidák multiple comparison test; n = 4.

Article Snippet: To test the role of SOCE, Fluo-4– or Fluo-5N–loaded platelets were treated with the Orai1 blocker Synta-66 (10 μM), stimulated with Thr/CRP-XL in the presence of AnV-APC, and analyzed by using flow cytometry.

Techniques: Flow Cytometry, Fluorescence, Comparison